2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

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RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-163325A
    Fli-1-IN-1
    		Inhibitor
    Fli-1-IN-1 (compound 21) is a FLI1 targeting agent that directly binds to and inhibits the EWS-FLI1 protein interaction. EWS-FLI1 can bind to RNA helicase A, and Fli-1-IN-1 inhibits EWS-FLI1 and has potential anticancer activity.
    Fli-1-IN-1
  • HY-157605
    DHX9-IN-11
    		Inhibitor
    DHX9-IN-11 (469) is a RNA helicase DHX9 inhibitor, with an EC50 of 0.0838 μM in DHX9 cellular target engagement. Used in cancer research.
    DHX9-IN-11
  • HY-149534
    DNA Gyrase-IN-9
    		Inhibitor
    DNA Gyrase-IN-9 (compound 4j) is an antibacterial agent that targets DNA gyrase. The MIC to inhibit Gram bacteria is 0.5-2 μg/mL, and the MBC to kill Gram bacteria is 2-8 μg/mL. DNA Gyrase-IN-9 inhibits DNA gyrase in Staphylococcus aureus with IC50=6.29 μg/mL.
    DNA Gyrase-IN-9
  • HY-W570890
    2'-O-MOE-5-Me-C(Bz)
    2'-O-MOE-5-Me-C (Bz) is a nucleotide for the stereoselective synthesis of nucleoside alkyl phosphonates.
    2'-O-MOE-5-Me-C(Bz)
  • HY-B1431A
    Butylparaben sodium
    		Inhibitor
    Butylparaben sodium strongly influences the later stages of the spermatogenesis in the testis through the deterioration of hormonal control and/or RNA and protein synthesis.
    Butylparaben sodium
  • HY-N1620
    1-Cinnamoylpyrrolidine
    1-Cinnamoylpyrrolidine (Compound 3), a crude extract prepared from Piper caninum, is a DNA strand scission agent, induces the relaxation of supercoiled pBR322 plasmid DNA. 1-Cinnamoylpyrrolidine (Compound 4) inhibits platelet aggregation induced by PAF with an IC50 of 37.3 μM.
    1-Cinnamoylpyrrolidine
  • HY-E70587
    exo-β-1,3-Xylosidase, Streptomyces sp. SWU10
    exo-β-1,3-Xylosidase, Streptomyces sp. SWU10 (EC.3.2.1.72) is an endo-xylanase that degrade xylans to several xylooligosaccharides.
    exo-β-1,3-Xylosidase, Streptomyces sp. SWU10
  • HY-E70376
    Pfu DNA Polymerase
    Pfu DNA Polymerase is an important enzyme in PCR-related experiments, which is initially found in Pyrococcus furiosus. Pfu DNA Polymerase exhibits thermostability and high fidelity.
    Pfu DNA Polymerase
  • HY-158670
    3'-NH2-ddGTP sodium
    3'-NH2-ddGTP sodium is a labeled modified deoxyoligonucleotide (dNTP) that can release pyrophosphate to produce fluorescence and has special applications in gene synthesis and sequencing.
    3'-NH2-ddGTP sodium
  • HY-W747737
    BVDU 5′-Triphosphate
    		Inhibitor
    BVDU 5′-Triphosphate is an antivirus agent with 5′-Triphosphate label, targeting viral DNA polymerase. BVDU 5′-Triphosphate shows excellent selectivity against varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1), due to a specific phosphorylation by the virus-encoded thymidine kinase.
    BVDU 5′-Triphosphate
  • HY-155094
    DNA polymerase-IN-3
    		Inhibitor
    DNA polymerase-IN-3 (Compd 5b) is a coumarin derivative that exhibits inhibitory activity against Taq DNA polymerase and can be used in proliferative disease research.
    DNA polymerase-IN-3
  • HY-W009538S
    5'-Deoxy-5-fluorocytidine-d3
    5'-Deoxy-5-fluorocytidine-d3 is deuterated labeled 5'-Deoxy-5-fluorocytidine (HY-W009538). 5'-Deoxy-5-fluorocytidine (5-Fluoro-5'-deoxycytidine) is a cytidine analog and metabolite of Capecitabine (HY-B0016). 5'-Deoxy-5-fluorocytidine is converted from Capecitabine by carboxylesterase in the liver. 5'-Deoxy-5-fluorocytidine is deaminated by cytidine deaminase to generate 5'-deoxy-5-fluorouridine, which is finally converted into 5-fluorouracil (HY-90006) by thymidine phosphorylase in tumor tissues to exert anti-tumor effects. 5'-Deoxy-5-fluorocytidine is used in the researches for solid tumors such as colorectal cancer, non-small cell lung cancer and breast cancer.
    5'-Deoxy-5-fluorocytidine-d<sub>3</sub>
  • HY-121777
    SFOM-0046
    		Inhibitor
    SFOM-0046 arrests cell cycle in S-phase and causes DNA replication stress leading to the phosphorylation of H2AX into γ-H2AX. SFOM-0046 induces DNA damages. SFOM-0046 is a potent anticancer agent.
    SFOM-0046
  • HY-W777340
    Gimeracil-13C3
    Gimeracil-13C3 (Gimestat-13C3) is the 13C-labeled Gimeracil (HY-17469). Gimeracil, a component of an oral fluoropyrimidine derivative S-1, inhibits DNA DSB repair and is a potent inhibitor of DPYD (dihydropyrimidine dehydrogenase, DPD).
    Gimeracil-<sup>13</sup>C<sub>3</sub>
  • HY-157608
    DHX9-IN-10
    		Inhibitor
    DHX9-IN-10 (49) is a RNA helicase DHX9 inhibitor, with an EC50 of 9.04 μM in DHX9 cellular target engagement. Used in cancer research.
    DHX9-IN-10
  • HY-120105
    NSC666715
    		Inhibitor
    NSC666715 is a strand-displacement activity of DNA polymerase inhibitor. NSC666715 potentiates Temozolomide (HY-17364)-induced DNA damage, cell senescence and apoptosis. NSC666715 can be used for study colorectal cancer.
    NSC666715
  • HY-163325
    Fli-1-IN-1 fumarate
    		Inhibitor
    Fli-1-IN-1 (compound 21) fumarate is a FLI1 targeting agent that directly binds to and inhibits the EWS-FLI1 protein interaction. EWS-FLI1 can bind to RNA helicase A, and Fli-1-IN-1 fumarate inhibits EWS-FLI1 and has potential anticancer activity.
    Fli-1-IN-1 fumarate
  • HY-125818S4
    Cytidine-5'-triphosphate-d14 dilithium
    		98.2%
    Cytidine-5'-triphosphate-d14 (Cytidine triphosphate-d14 dilithium; 5'-CTP-d14) dilithium is deuterium labeled Cytidine-5'-triphosphate (HY-125818). Cytidine 5′-triphosphate (Cytidine triphosphate; 5'-CTP) is a nucleoside triphosphate and serves as a building block for nucleotides and nucleic acids, lipid biosynthesis. Cytidine triphosphate synthase can catalyze the formation of cytidine 5′-triphosphate from uridine 5′-triphosphate (UTP). Cytidine 5′-triphosphate is an essential biomolecule in the de novo pyrimidine biosynthetic pathway in T. gondii.
    Cytidine-5'-triphosphate-d<sub>14</sub> dilithium
  • HY-150330
    DNA ligase-IN-1
    		Inhibitor
    DNA ligase-IN-1 (Compound 1) is an inhibitor of bacterial NAD+-dependent DNA ligase (LigA). DNA ligase-IN-1 effectively inhibits the growth of Staphylococcus aureus in vitro.
    DNA ligase-IN-1
  • HY-154989
    RdRP-IN-6
    		Inhibitor
    RdRP-IN-6 (compound 27) inhibits RNA dependent-RNA polymerase (RdRp) with an IC90 value of 14.1 μM. RdRP-IN-6 can be used for research on antiviral and anticancer.
    RdRP-IN-6
Cat. No. Product Name / Synonyms Application Reactivity